Reverse Transcriptase PCR Amplification of Rickettsia typhi from Infected Mammalian Cells and Insect Vectors
نویسندگان
چکیده
منابع مشابه
Reverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors.
We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was amplified by using high concentrations of primer and template in an inexpensive, nonradioactive assay.
متن کاملPCR - Based Reverse Transcriptase Assay
ES cells. This phenomenon has been observed in our laboratory in a number of cases. Clones putatively identified as homologous recombinants by Southern analysis can be quickly checked for random insertion of extra copies of the targeting vector by PCR. Given the small difference in size between the two alleles and use of only two primers common to both WT and mutant DNA, the amplification rate ...
متن کاملRecombinant baculoviruses as expression vectors for insect and mammalian cells.
Baculovirus expression vectors are widely used for expressing heterologous proteins in cultured insect cells. Recent advances include further development of the system for production of multi-subunit protein complexes, co-expression of protein-modifying enzymes to improve heterologous protein production, and additional applications of baculovirus display technology. The application of modified ...
متن کاملPCR Amplification and Sequence Analyses of Reverse Transcriptase- like Genes in Crinipellis perniciosa Isolates
Reverse transcriptase (RT) sequence analysis is an important technique used to detect the presence of transposable elements in a genome. Putative RT sequences were analyzed in the genome of the pathogenic fungus C. perniciosa, the causal agent of witches’ broom disease of cocoa. A 394 bp fragment was amplified from genomic DNA of different isolates of C. perniciosa belonging to C-, L-, and S-bi...
متن کاملIsolation of Rickettsia typhi from Human, Mexico
To the Editor: Murine typhus is a febrile illness caused by Rickettsia typhi. The clinical manifestations are nonspecific, and the signs and symptoms resemble those of several other febrile illnesses. Murine typhus can be a self-limiting infection; however, it should be diagnosed and treated because complications and even death can result (1). In Mexico, particularly in Yucatan State, cases of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1998
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.36.6.1793-1794.1998